Engineering and Evolution of Molecular Chaperones and Protein Disaggregases with Enhanced Activity.

ABSTRACT: Cells have evolved a sophisticated proteostasis network to ensure that proteins acquire and retain their native structure and function. Critical components of this network include molecular chaperones and protein disaggregases, which function to prevent and reverse deleterious protein misfolding. Nevertheless, proteostasis networks have limits, which when exceeded can have fatal consequences as in various neurodegenerative disorders, including Parkinson's disease and amyotrophic lateral sclerosis. A promising strategy is to engineer proteostasis networks to counter challenges presented by specific diseases or specific proteins. Here, we review efforts to enhance the activity of individual molecular chaperones or protein disaggregases via engineering and directed evolution. Remarkably, enha

SUBMITTER: Mack KL 

PROVIDER: S-EPMC4791398 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature