Developing informative microsatellite markers for non-model species using reference mapping against a model species' genome.

ABSTRACT: Microsatellites have a wide range of applications from behavioral biology, evolution, to agriculture-based breeding programs. The recent progress in the next-generation sequencing technologies and the rapidly increasing number of published genomes may greatly enhance the current applications of microsatellites by turning them from anonymous to informative markers. Here we developed an approach to anchor microsatellite markers of any target species in a genome of a related model species, through which the genomic locations of the markers, along with any functional genes potentially linked to them, can be revealed. We mapped the shotgun sequence reads of a non-model rodent species Apodemus semotus against the genome of a model species, Mus musculus, and presented 24 polymorphic microsatellit

SUBMITTER: Hung CM 

PROVIDER: S-EPMC4791680 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature