Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.

ABSTRACT:

Background

The saliva of tsetse flies contains a cocktail of bioactive molecules inducing specific antibody responses in hosts exposed to bites. We have previously shown that an indirect-ELISA test using whole salivary extracts from Glossina morsitans submorsitans was able to discriminate between (i) cattle from tsetse infested and tsetse free areas and (ii) animals experimentally exposed to low or high numbers of tsetse flies. In the present study, our aim was to identify specific salivary synthetic peptides that could be used to develop simple immunoassays to measure cattle exposure to tsetse flies.

Methods

In a first step, 2D-electrophoresis immunoblotting, using sera from animals exposed to a variety of bloodsucking arthropods, was performed to identify specific salivary

SUBMITTER: Somda MB 

PROVIDER: S-EPMC4791801 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature