Rapid gene identification in sugar beet using deep sequencing of DNA from phenotypic pools selected from breeding panels.

ABSTRACT:

Background

The combination of bulk segregant analysis (BSA) and next generation sequencing (NGS), also known as mapping by sequencing (MBS), has been shown to significantly accelerate the identification of causal mutations for species with a reference genome sequence. The usual approach is to cross homozygous parents that differ for the monogenic trait to address, to perform deep sequencing of DNA from F2 plants pooled according to their phenotype, and subsequently to analyze the allele frequency distribution based on a marker table for the parents studied. The method has been successfully applied for EMS induced mutations as well as natural variation. Here, we show that pooling genetically diverse breeding lines according to a contrasting phenotype also allows high resolution mapp

SUBMITTER: Ries D 

PROVIDER: S-EPMC4791833 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature