Single-cell transcriptome analysis of endometrial tissue.

ABSTRACT:

Study question

How can we study the full transcriptome of endometrial stromal and epithelial cells at the single-cell level?

Summary answer

By compiling and developing novel analytical tools for biopsy, tissue cryopreservation and disaggregation, single-cell sorting, library preparation, RNA sequencing (RNA-seq) and statistical data analysis.

What is known already

Although single-cell transcriptome analyses from various biopsied tissues have been published recently, corresponding protocols for human endometrium have not been described.

Study design, size, duration

The frozen-thawed endometrial biopsies were fluorescence-activated cell sorted (FACS) to distinguish CD13-positive stromal and CD9-positive epithelial cells and single-cell transcriptome analysis perf

SUBMITTER: Krjutskov K 

PROVIDER: S-EPMC4791917 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature