Nanoscale electron transport measurements of immobilized cytochrome P450 proteins.

ABSTRACT: Gold nanopillars, functionalized with an organic self-assembled monolayer, can be used to measure the electrical conductance properties of immobilized proteins without aggregation. Measurements of the conductance of nanopillars with cytochrome P450 2C9 (CYP2C9) proteins using conducting probe atomic force microscopy demonstrate that a correlation exists between the energy barrier height between hopping sites and CYP2C9 metabolic activity. Measurements performed as a function of tip force indicate that, when subjected to a large force, the protein is more stable in the presence of a substrate. This agrees with the hypothesis that substrate entry into the active site helps to stabilize the enzyme. The relative distance between hopping sites also increases with increasing force, possibly beca

SUBMITTER: Bostick CD 

PROVIDER: S-EPMC4791957 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature