Differential Regulation of Progesterone Receptor-Mediated Transcription by CDK2 and DNA-PK.

ABSTRACT: Progesterone receptor (PR) function is altered by cell signaling, but the mechanisms of kinase-specific regulation are not well defined. To examine the role of cell signaling in the regulation of PR transcriptional activity, we have utilized a previously developed mammalian-based estrogen-response element promoter array cell model and automated cell imaging and analysis platform to visualize and quantify effects of specific kinases on different mechanistic steps of PR-mediated target gene activation. For these studies, we generated stable estrogen-response element array cell lines expressing inducible chimeric PR that contains a swap of the estrogen receptor-α DNA-binding domain for the DNA-binding domain of PR. We have focused on 2 kinases important for steroid receptor activity: cyclin-d

SUBMITTER: Trevino LS 

PROVIDER: S-EPMC4792227 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature