Genomic responses to hepatitis B virus (HBV) infection in primary human hepatocytes.

ABSTRACT: Viral infections are able to modify the host's cellular programs, with DNA methylation being a biological intermediate in this process. The extent to which viral infections deregulate gene expression and DNA methylation is not fully understood. In the case of Hepatitis B virus (HBV), there is evidence for an interaction between viral proteins and the host DNA methylation machinery. We studied the ability of HBV to modify the host transcriptome and methylome, using naturally infected primary human hepatocytes to better mimic the clinical setting.Gene expression was especially sensitive to culture conditions, independently of HBV infection. However, we identified non-random changes in gene expression and DNA methylation occurring specifically upon HBV infection. There was little correlation

SUBMITTER: Ancey PB 

PROVIDER: S-EPMC4792598 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature