Comprehensive untargeted lipidomic analysis using core-shell C30 particle column and high field orbitrap mass spectrometer.

ABSTRACT: The goal of untargeted lipidomics is to have high throughput, yet comprehensive and unambiguous identification and quantification of lipids. Novel stationary phases in LC separation and new mass spectrometric instruments capable of high mass resolving power and faster scanning rate are essential to achieving this goal. In this work, 4 reversed phase LC columns coupled with a high field quadrupole orbitrap mass spectrometer (Q Exactive HF) were thoroughly compared using complex lipid standard mixture and rat plasma and liver samples. A good separation of all lipids was achieved in 24min of gradient. The columns compared include C30 and C18 functionalization on either core-shell or totally porous silica particles, with size ranging from 1.7 to 2.6μm. Accucore C30 column showed the narrowest

SUBMITTER: Narvaez-Rivas M 

PROVIDER: S-EPMC4792668 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature