Display of GPI-anchored anti-EGFR nanobodies on extracellular vesicles promotes tumour cell targeting.

ABSTRACT:

Background

Extracellular vesicles (EVs) are attractive candidate drug delivery systems due to their ability to functionally transport biological cargo to recipient cells. However, the apparent lack of target cell specificity of exogenously administered EVs limits their therapeutic applicability. In this study, we propose a novel method to equip EVs with targeting properties, in order to improve their interaction with tumour cells.

Methods

EV producing cells were transfected with vectors encoding for anti-epidermal growth factor receptor (EGFR) nanobodies, which served as targeting ligands for tumour cells, fused to glycosylphosphatidylinositol (GPI) anchor signal peptides derived from decay-accelerating factor (DAF). EVs were isolated using ultrafiltration/size-exclusion liq

SUBMITTER: Kooijmans SA 

PROVIDER: S-EPMC4793259 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature