Early detection of novel Leishmania species DNA in the saliva of two HIV-infected patients.

ABSTRACT:

Background

Leishmaniasis caused by two new species of Leishmania; L. siamensis and L. martiniquensis have been recently described in Thailand. The disease has mainly been documented in AIDS patients from southern Thailand. In this study, polymerase chain reaction (PCR) was used to determine HIV-Leishmania co-infection in southern Thailand.

Methods

One ml of saliva and 3 ml of EDTA blood were collected from HIV-infected patients for PCR detection of Leishmania DNA, cloning and sequencing. The positive PCR samples were then cultured on Schneider's insect medium.

Results

Three out of 316 saliva samples collected from HIV-infected patients were found to be positive for Leishmania DNA (0.95%). Among the positive samples, one patient was observed with disseminated cutaneous

SUBMITTER: Siriyasatien P 

PROVIDER: S-EPMC4793580 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature