Modulation of anti-cancer drug sensitivity through the regulation of mitochondrial activity by adenylate kinase 4.

ABSTRACT:

Background

Adenylate kinase is a key enzyme in the high-energy phosphoryl transfer reaction in living cells. An isoform of this enzyme, adenylate kinase 4 (AK4), is localized in the mitochondrial matrix and is believed to be involved in stress, drug resistance, malignant transformation in cancer, and ATP regulation. However, the molecular basis for the AK4 functions remained to be determined.

Methods

HeLa cells were transiently transfected with an AK4 small interfering RNA (siRNA), an AK4 short hairpin RNA (shRNA) plasmid, a control shRNA plasmid, an AK4 expression vector, and a control expression vector to examine the effect of the AK4 expression on cell proliferation, sensitivity to anti-cancer drug, metabolome, gene expression, and mitochondrial activity.

Results

A

SUBMITTER: Fujisawa K 

PROVIDER: S-EPMC4793738 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature