A model to study the phenotypic changes of interstitial cells of Cajal in gastrointestinal diseases.

ABSTRACT:

Background & aims

Interstitial cells of Cajal (ICC) express the receptor tyrosine kinase, KIT, the receptor for stem cell factor. In the gastrointestinal (GI) tract, ICC are pacemaker cells that generate spontaneous electrical slow waves, and mediate inputs from motor neurons. Absence or loss of ICC are associated with GI motility disorders, including those consequent of diabetes. Studies of ICC have been hampered by the low density of these cells and difficulties in recognizing these cells in cell dispersions.

Methods

Kit(+/copGFP) mice harboring a copepod super green fluorescent protein (copGFP) complementary DNA, inserted at the Kit locus, were generated. copGFP(+) ICC from GI muscles were analyzed using confocal microscopy and flow cytometry. copGFP(+) ICC from the jejun

SUBMITTER: Ro S 

PROVIDER: S-EPMC4793910 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature