The Intestinal Eukaryotic Virome in Healthy and Diarrhoeic Neonatal Piglets.

ABSTRACT: Neonatal porcine diarrhoea of uncertain aetiology has been reported from a number of European countries. The aim of the present study was to use viral metagenomics to examine a potential viral involvement in this diarrhoea and to describe the intestinal virome with focus on eukaryotic viruses. Samples from the distal jejunum of 50 diarrhoeic and 19 healthy piglets from 10 affected herds were analysed. The viral fraction of the samples was isolated and nucleic acids (RNA and DNA fractions) were subjected to sequence independent amplification. Samples from diarrhoeic piglets from the same herds were pooled whereas samples from healthy piglets were analysed individually. In total, 29 clinical samples, plus two negative controls and one positive control consisting of a mock metagenome were seq

SUBMITTER: Karlsson OE 

PROVIDER: S-EPMC4794121 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature