Quantitation and Identification of Thousands of Human Proteoforms below 30 kDa.

ABSTRACT: Top-down proteomics is capable of identifying and quantitating unique proteoforms through the analysis of intact proteins. We extended the coverage of the label-free technique, achieving differential analysis of whole proteins <30 kDa from the proteomes of growing and senescent human fibroblasts. By integrating improved control software with more instrument time allocated for quantitation of intact ions, we were able to collect protein data between the two cell states, confidently comparing 1577 proteoform levels. To then identify and characterize proteoforms, our advanced acquisition software, named Autopilot, employed enhanced identification efficiency in identifying 1180 unique Swiss-Prot accession numbers at 1% false-discovery rate. This coverage of the low mass proteome is equivalent

SUBMITTER: Durbin KR 

PROVIDER: S-EPMC4794255 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature