A new hand-held microfluidic cytometer for evaluating irradiation damage by analysis of the damaged cells distribution.

ABSTRACT: Space radiation brings uneven damages to cells. The detection of the distribution of cell damage plays a very important role in radiation medicine and the related research. In this paper, a new hand-held microfluidic flow cytometer was developed to evaluate the degree of radiation damage of cells. The device we propose overcomes the shortcomings (e.g., large volume and high cost) of commercial flow cytometers and can evaluate the radiation damage of cells accurately and quickly with potential for onsite applications. The distribution of radiation-damaged cells is analyzed by a simultaneous detection of immunofluorescence intensity of γ-H2AX and resistance pulse sensor (RPS) signal. The γ-H2AX fluorescence intensity provides information of the degree of radiation damage in cells. The ratio

SUBMITTER: Wang J 

PROVIDER: S-EPMC4794725 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature