Molecular detection and identification of piroplasms in sika deer (Cervus nippon) from Jilin Province, China.

ABSTRACT:

Background

Piroplasmosis is an important disease of domestic animals and wildlife and is caused by organisms from the genera Theileria and Babesia. Wildlife such as sika deer play an important role as reservoir hosts for several species of Theileria and Babesia. Using blood samples collected from sika deer, we investigated the epidemiology of Theileria spp. and Babesia spp. in sika deer from Jilin Province in China and identified those species that cause pathogenic infections in sika deer.

Methods

Sixty-eight blood samples of sika deer were collected from three areas of the Jilin Province in Northeast China. Genomic DNA was extracted, and the V4 hypervariable region of the 18S rRNA of the piroplasms was amplified using the nested PCR method. The selected positive samples wer

SUBMITTER: Liu J 

PROVIDER: S-EPMC4794910 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature