Determinants of protein elution rates from preparative ion-exchange adsorbents.

ABSTRACT: The rate processes involved in elution in preparative chromatography can affect both peak resolution and hence selectivity as well as practical factors such as facility fit. These processes depend on the physical structure of the adsorbent particles, the amount of bound solute, the solution conditions for operation or some combination of these factors. Ion-exchange adsorbents modified with covalently attached or grafted polymer layers have become widely used in preparative chromatography. Their often easily accessible microstructures offer substantial binding capacities for biomolecules, but elution has sometimes been observed to be undesirably slow. In order to determine which physicochemical phenomena control elution behavior, commercially available cellulosic, dextran-grafted and unmodi

SUBMITTER: Angelo JM 

PROVIDER: S-EPMC4795180 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature