Distinct metabolism of apolipoproteins (a) and B-100 within plasma lipoprotein(a).

ABSTRACT:

Objectives

Lipoprotein(a) [Lp(a)] is mainly similar in composition to LDL, but differs in having apolipoprotein (apo) (a) covalently linked to apoB-100. Our purpose was to examine the individual metabolism of apo(a) and apoB-100 within plasma Lp(a).

Materials and methods

The kinetics of apo(a) and apoB-100 in plasma Lp(a) were assessed in four men with dyslipidemia [Lp(a) concentration: 8.9-124.7nmol/L]. All subjects received a primed constant infusion of [5,5,5-(2)H3] L-leucine while in the constantly fed state. Lp(a) was immunoprecipitated directly from whole plasma; apo(a) and apoB-100 were separated by gel electrophoresis; and isotopic enrichment was determined by gas chromatography/mass spectrometry.

Results

Multicompartmental modeling analysis indicated that the

SUBMITTER: Diffenderfer MR 

PROVIDER: S-EPMC4795479 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature