Drug-set enrichment analysis: a novel tool to investigate drug mode of action.

ABSTRACT:

Motivation

Automated screening approaches are able to rapidly identify a set of small molecules inducing a desired phenotype from large small-molecule libraries. However, the resulting set of candidate molecules is usually very diverse pharmacologically, thus little insight on the shared mechanism of action (MoA) underlying their efficacy can be gained.

Results

We introduce a computational method (Drug-Set Enrichment Analysis-DSEA) based on drug-induced gene expression profiles, which is able to identify the molecular pathways that are targeted by most of the drugs in the set. By diluting drug-specific effects unrelated to the phenotype of interest, DSEA is able to highlight phenotype-specific pathways, thus helping to formulate hypotheses on the MoA shared by the drugs in t

SUBMITTER: Napolitano F 

PROVIDER: S-EPMC4795590 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature