PopulationProfiler: A Tool for Population Analysis and Visualization of Image-Based Cell Screening Data.

ABSTRACT: Image-based screening typically produces quantitative measurements of cell appearance. Large-scale screens involving tens of thousands of images, each containing hundreds of cells described by hundreds of measurements, result in overwhelming amounts of data. Reducing per-cell measurements to the averages across the image(s) for each treatment leads to loss of potentially valuable information on population variability. We present PopulationProfiler-a new software tool that reduces per-cell measurements to population statistics. The software imports measurements from a simple text file, visualizes population distributions in a compact and comprehensive way, and can create gates for subpopulation classes based on control samples. We validate the tool by showing how PopulationProfiler can be u

SUBMITTER: Matuszewski DJ 

PROVIDER: S-EPMC4795740 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature