Identification and characterization of microsatellite markers in Penstemon scariosus (Plantaginaceae).

ABSTRACT: Penstemon scariosus var. albifluvis (Plantaginaceae) has been proposed to be federally listed as threatened due to its unique, geologically oil-rich habitat. Developing simple sequence repeat (SSR) markers to study its genetic diversity would be most useful. Using genomic reduction in combination with next-generation sequencing, we identified SSR motifs with five to 15 perfect repeats in 1067 P. scariosus contigs. After multiple qualifying tests, 16 SSRs were selected for their robust polymorphic reliability across 12 taxa with as high as 21 alleles in a given taxon. With the exception of two monomorphic loci, the observed and expected heterozygosity values ranged from 0.083 to 1.000 and 0.398 to 0.920, respectively. These microsatellite markers will directly aid in studies of the genetic

SUBMITTER: Anderson CD 

PROVIDER: S-EPMC4795917 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature