R-Syst::diatom: an open-access and curated barcode database for diatoms and freshwater monitoring.

ABSTRACT: Diatoms are micro-algal indicators of freshwater pollution. Current standardized methodologies are based on microscopic determinations, which is time consuming and prone to identification uncertainties. The use of DNA-barcoding has been proposed as a way to avoid these flaws. Combining barcoding with next-generation sequencing enables collection of a large quantity of barcodes from natural samples. These barcodes are identified as certain diatom taxa by comparing the sequences to a reference barcoding library using algorithms. Proof of concept was recently demonstrated for synthetic and natural communities and underlined the importance of the quality of this reference library. We present an open-access and curated reference barcoding database for diatoms, called R-Syst::diatom, developed i

SUBMITTER: Rimet F 

PROVIDER: S-EPMC4795936 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature