Instrumentation to study myofibril mechanics from static to artificial simulations of cardiac cycle.

ABSTRACT: Many causes of heart muscle diseases and skeletal muscle diseases are inherited and caused by mutations in genes of sarcomere proteins which play either a structural or contractile role in the muscle cell. Tissue samples from human hearts with mutations can be obtained but often samples are only a few milligrams and it is necessary to freeze them for storage and transportation. Myofibrils are the fundamental contractile components of the muscle cell and retain all structural elements and contractile proteins performing in contractile event; moreover viable myofibrils can be obtained from frozen tissue.•We are describing a versatile technique for measuring the contractility and its Ca(2+) regulation in single myofibrils. The control of myofibril length, incubation medium and data acquisitio

SUBMITTER: Vikhorev PG 

PROVIDER: S-EPMC4796715 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature