Comparative analysis of bacterial profiles in unstimulated and stimulated saliva samples.

ABSTRACT:

Background and objective

The microbial profiles of stimulated saliva samples have been shown to differentiate between patients with periodontitis, patients with dental caries, and orally healthy individuals. Saliva was stimulated to allow for easy and rapid collection; however, microbial composition may not reflect the more natural, unstimulated state. The purpose of this study was to validate whether stimulated saliva is an adequate surrogate for unstimulated saliva in determining salivary microbiomes.

Design

Unstimulated (n=20) and stimulated (n=20) saliva samples were collected from 20 orally and systemically healthy, non-smoking participants. Salivary bacterial profiles were analyzed by means of the Human Oral Microbe Identification using Next Generation Sequencing (HOMI

SUBMITTER: Belstrom D 

PROVIDER: S-EPMC4796727 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature