Proteome analysis of the macroscopically affected colonic mucosa of Crohn's disease and intestinal tuberculosis.

ABSTRACT: Differentiation between intestinal tuberculosis (ITB) and Crohn's disease (CD) is challenging in geographical regions where both these diseases are prevalent. There is a need of biomarkers for differentiation between these two disorders. Colonic biopsies from inflamed mucosa of treatment-naive patients with ITB, CD and controls were used for analysis. Protein extracted from biopsies was digested with trypsin and resulting peptides were labeled with iTRAQ reagents. The peptides were subsequently analyzed using LC-MS/MS for identification and quantification. Gene ontology annotation for proteins was analyzed in PANTHER. Validation experiments were done for six differentially expressed proteins using immunohistochemistry. 533 proteins were identified and 241 proteins were quantified from 5 se

SUBMITTER: Rukmangadachar LA 

PROVIDER: S-EPMC4796817 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature