Metatranscriptomic insights on gene expression and regulatory controls in Candidatus Accumulibacter phosphatis.

ABSTRACT: Previous studies on enhanced biological phosphorus removal (EBPR) have focused on reconstructing genomic blueprints for the model polyphosphate-accumulating organism Candidatus Accumulibacter phosphatis. Here, a time series metatranscriptome generated from enrichment cultures of Accumulibacter was used to gain insight into anerobic/aerobic metabolism and regulatory mechanisms within an EBPR cycle. Co-expressed gene clusters were identified displaying ecologically relevant trends consistent with batch cycle phases. Transcripts displaying increased abundance during anerobic acetate contact were functionally enriched in energy production and conversion, including upregulation of both cytoplasmic and membrane-bound hydrogenases demonstrating the importance of transcriptional regulation to mana

SUBMITTER: Oyserman BO 

PROVIDER: S-EPMC4796919 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature