Viability of Pathologic Cartilage Fragments as a Source for Autologous Chondrocyte Cultures.

ABSTRACT:

Objective

To study if a culture of chondrocytes can be obtained from pathologic hyaline cartilage (PHC) fragments.

Design

Twenty-five men and 9 women with osteochondritis dissecans (OCD) in 11 cases, arthrosis in 13 patients, and trauma in the remaining 10 cases were included. The PHC fragments and a small sample of the next healthy cartilage were extracted by arthroscopy. According to the appearance, the PHC samples were divided into fixed (3 cases), flapped (6 patients), or loose bodies (25 cases), depending on the attachment degree of the cartilage to the subchondral bone. Approximately half of each pathologic sample and the whole healthy one were digested to isolate the cells trying to establish the cell culture.

Results

We were able to establish a cell culture in

SUBMITTER: Guillen-Garcia P 

PROVIDER: S-EPMC4797241 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature