RING1A and BMI1 bookmark active genes via ubiquitination of chromatin-associated proteins.

ABSTRACT: During mitosis the chromatin undergoes dramatic architectural changes with the halting of the transcriptional processes and evacuation of nearly all transcription associated machinery from genes and promoters. Molecular bookmarking of genes during mitosis is a mechanism of faithfully transmitting cell-specific transcription patterns through cell division. We previously discovered chromatin ubiquitination at active promoters as a potential mitotic bookmark. In this study, we identify the enzymes involved in the deposition of ubiquitin before mitosis. We find that the polycomb complex proteins BMI1 and RING1A regulate the ubiquitination of chromatin associated proteins bound to promoters, and this modification is necessary for the expression of marked genes once the cells enter G1. Depletion

SUBMITTER: Arora M 

PROVIDER: S-EPMC4797268 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature