Transcription profile of Escherichia coli: genomic SELEX search for regulatory targets of transcription factors.

ABSTRACT: Bacterial genomes are transcribed by DNA-dependent RNA polymerase (RNAP), which achieves gene selectivity through interaction with sigma factors that recognize promoters, and transcription factors (TFs) that control the activity and specificity of RNAP holoenzyme. To understand the molecular mechanisms of transcriptional regulation, the identification of regulatory targets is needed for all these factors. We then performed genomic SELEX screenings of targets under the control of each sigma factor and each TF. Here we describe the assembly of 156 SELEX patterns of a total of 116 TFs performed in the presence and absence of effector ligands. The results reveal several novel concepts: (i) each TF regulates more targets than hitherto recognized; (ii) each promoter is regulated by more TFs than

SUBMITTER: Ishihama A 

PROVIDER: S-EPMC4797297 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature