Glioma-derived extracellular vesicles selectively suppress immune responses.

ABSTRACT:

Background

Glioma-related immunosuppression is well documented; however, the mechanisms of suppression are not fully understood. Here we explore a role for glioma extracellular vesicles (EVs) as a means of immune modulation.

Methods

Healthy donor peripheral blood mononuclear cells (PBMCs) were incubated with mitogenic stimuli and various concentrations of glioma-derived EVs. Intracellular signaling and cytokine output were determined by protein microarrays, and phenotypic changes were assessed by flow cytometry. Recall antigen testing, mixed lymphocyte reactions, and migration assays analyzed PBMC functional capacity.

Results

Protein microarray data revealed induction of an immunosuppressive phenotype and cytokine output at high tumor-vesicle concentrations but an act

SUBMITTER: Hellwinkel JE 

PROVIDER: S-EPMC4799676 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature