Lighting Up Clostridium Difficile: Reporting Gene Expression Using Fluorescent Lov Domains.

ABSTRACT: The uses of fluorescent reporters derived from green fluorescent protein have proved invaluable for the visualisation of biological processes in bacteria grown under aerobic conditions. However, their requirement for oxygen has limited their application in obligate anaerobes such as Clostridium difficile. Fluorescent proteins derived from Light, Oxygen or Voltage sensing (LOV) domains have been shown to bridge this limitation, but their utility as translational fusions to monitor protein expression and localisation in a strict anaerobic bacterium has not been reported. Here we demonstrate the utility of phiLOV in three species of Clostridium and its application as a marker of real-time protein translation and dynamics through genetic fusion with the cell division protein, FtsZ. Time lapse

SUBMITTER: Buckley AM 

PROVIDER: S-EPMC4800718 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature