Metagenomic analysis of bloodstream infections in patients with acute leukemia and therapy-induced neutropenia.

ABSTRACT: Leukemic patients are often immunocompromised due to underlying conditions, comorbidities and the effects of chemotherapy, and thus at risk for developing systemic infections. Bloodstream infection (BSI) is a severe complication in neutropenic patients, and is associated with increased mortality. BSI is routinely diagnosed with blood culture, which only detects culturable pathogens. We analyzed 27 blood samples from 9 patients with acute leukemia and suspected BSI at different time points of their antimicrobial treatment using shotgun metagenomics sequencing in order to detect unculturable and non-bacterial pathogens. Our findings confirm the presence of bacterial, fungal and viral pathogens alongside antimicrobial resistance genes. Decreased white blood cell (WBC) counts were associated w

SUBMITTER: Gyarmati P 

PROVIDER: S-EPMC4800731 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature