Short-Stalked Prosthecomicrobium hirschii Cells Have a Caulobacter-Like Cell Cycle.

ABSTRACT:

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The dimorphic alphaproteobacterium Prosthecomicrobium hirschii has both short-stalked and long-stalked morphotypes. Notably, these morphologies do not arise from transitions in a cell cycle. Instead, the maternal cell morphology is typically reproduced in daughter cells, which results in microcolonies of a single cell type. In this work, we further characterized the short-stalked cells and found that these cells have a Caulobacter-like life cycle in which cell division leads to the generation of two morphologically distinct daughter cells. Using a microfluidic device and total internal reflection fluorescence (TIRF) microscopy, we observed that motile short-stalked cells attach to a surface by means of a polar adhesin. Cells attached at their poles elongate and ultimatel

SUBMITTER: Williams M 

PROVIDER: S-EPMC4800870 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature