Regionally Discrete Aqueous Humor Outflow Quantification Using Fluorescein Canalograms.

ABSTRACT:

Purpose

To visualize and quantify conventional outflow directly in its anatomic location.

Methods

We obtained fluorescein canalograms in six porcine whole eyes and six porcine anterior segment cultures. Eyes were perfused with a constant pressure of 15 mmHg using media containing 0.017 mg/ml fluorescein. Flow patterns were visualized using a stereo dissecting microscope equipped for fluorescent imaging. Images were captured every 30 seconds for 20 minutes for time lapse analysis. Anterior chamber cultures were imaged again on day three of culture. Canalograms were first analyzed for filling time per quadrant. We then wrote a program to automatically compute focal flow fits for each macropixel and to detect convergent perilimbal flow patterns with macropixels grouped into 3 e

SUBMITTER: Loewen RT 

PROVIDER: S-EPMC4801333 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature