CsoR Is Essential for Maintaining Copper Homeostasis in Mycobacterium tuberculosis.

ABSTRACT: Mycobacterium tuberculosis, a pathogen infecting one third of the world population, faces numerous challenges within the host, including high levels of copper. We have previously shown that M. tuberculosis CsoR is a copper inducible transcriptional regulator. Here we examined the hypothesis that csoR is necessary for maintaining copper homeostasis and surviving under various stress conditions. With an unmarked csoR knockout strain, we were able to characterize the role of csoR in M. tuberculosis as it faced copper and host stress. Growth under high levels of copper demonstrated that M. tuberculosis survives copper stress significantly better in the absence of csoR. Yet under minimal levels of copper, differential expression analysis revealed that the loss of csoR results in a cell wide hyp

SUBMITTER: Marcus SA 

PROVIDER: S-EPMC4801387 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature