A flow cytometric approach to analyzing mature and progenitor endothelial cells following traumatic brain injury.

ABSTRACT:

Background

Traumatic brain injury (TBI) continues to be a major source of death and disability worldwide, and one of the earliest and most profound deficits comes from vascular damage and breakdown of the blood-brain barrier (BBB). Cerebral vascular endothelial cells (cvECs) and endothelial progenitor cells (EPCs) have been shown to play essential roles in vessel repair and BBB stability, although their individual contributions remain poorly defined.

New method

We employ TruCount beads with flow cytometry to precisely quantify cvECs, EPCs, and peripheral leukocytes in the murine cortex after controlled cortical impact (CCI) injury.

Results

We found a significant reduction in the number of cvECs at 3 days post-injury (dpi), whereas the EPCs and invading peripheral leuk

SUBMITTER: Assis-Nascimento P 

PROVIDER: S-EPMC4801778 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature