The isolation and molecular characterization of Mycobacterium avium subsp. paratuberculosis in Shandong province, China.

ABSTRACT:

Background

Mycobacterium avium subspecies paratuberculosis (Map) causes Johne's disease in domestic and wild ruminants. It has been a debate that whether Map can cause Crohn's disease in human. To our knowledge there is no report about molecular characterization of Map in China, although several Map strains have been reported in other country. The objectives of this study was to know the recent prevalence of Johne's disease in dairy farms in Shandong province, and have a better understanding of genotypic distribution of Map in China.

Methods

Johne's disease was detected from 1038 individuals in 19 dairy farms by ELISA. Map in fecal and milk specimens was identified by Ziehl-Neelsen staining and confirmed using PCR-REA. In addition, frozen sections of ileum and mesenteric lym

SUBMITTER: Yue R 

PROVIDER: S-EPMC4802609 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature