Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics.

ABSTRACT:

Background

Plant viruses in the genus Begomovirus, family Geminiviridae often cause substantial crop losses. These viruses have been emerging in many locations throughout the tropics and subtropics. Like many plant viruses, they are often not recognized by plant diagnostic clinics due in large part to the lack of rapid and cost effective assays. An isothermal amplification assay, Recombinase polymerase amplification (RPA), was evaluated for its ability to detect three begomoviruses and for its suitability for use in plant diagnostic clinics. Methods for DNA extraction and separation of amplicons from proteins used in the assay were modified and compared to RPA manufacturer's protocols. The modified RPA assays were compared to PCR assays for sensitivity, use in downstream applicatio

SUBMITTER: Londono MA 

PROVIDER: S-EPMC4802622 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature