Genetic characterization of OVC8 lytic phage for Vibrio cholerae O1.

ABSTRACT:

Background

Epidemics and pandemics of cholera, a diarrheal disease, are attributed to Vibrio cholera serogroups O1 and O139. In recent years, specific lytic phages of V. cholera have been proposed to be important factors in the cyclic occurrence of cholera in endemic areas. However, the role and potential participation of lytic phages during long interepidemic periods of cholera in non-endemic regions have not yet been described. The purpose of this study was to isolate and characterize specific lytic phages of V. cholera O1 strains.

Methods

Sixteen phages were isolated from wastewater samples collected at the Endhó Dam in Hidalgo State, Mexico, concentrated with PEG/NaCl, and purified by density gradient. The lytic activity of the purified phages was tested using different

SUBMITTER: Solis-Sanchez A 

PROVIDER: S-EPMC4802629 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature