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Identification and functional analyses of sex determination genes in the sexually dimorphic stag beetle Cyclommatus metallifer.


ABSTRACT:

Background

Genes in the sex determination pathway are important regulators of sexually dimorphic animal traits, including the elaborate and exaggerated male ornaments and weapons of sexual selection. In this study, we identified and functionally analyzed members of the sex determination gene family in the golden metallic stag beetle Cyclommatus metallifer, which exhibits extreme differences in mandible size between males and females.

Results

We constructed a C. metallifer transcriptomic database from larval and prepupal developmental stages and tissues of both males and females. Using Roche 454 pyrosequencing, we generated a de novo assembled database from a total of 1,223,516 raw reads, which resulted in 14,565 isotigs (putative transcript isoforms) contained in 10,794 isog

SUBMITTER: Gotoh H 

PROVIDER: S-EPMC4802893 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature