Molecular Characterization of Giardia duodenalis in Children in Kenya.

ABSTRACT:

Background

Giardia duodenalis is an important intestinal protozoan in humans worldwide with high infection rates occurring in densely populated and low resource settings. The parasite has been recorded to cause diarrhea in children. This study was carried out to identify G. duodenalis assemblages and sub-assemblages in children presenting with diarrhea in Kenya.

Methods

A total of 2112 faecal samples were collected from children aged ≤ 5 years and screened for the presence of Giardia cysts using microscopy. A total of 96 (4.5%) samples were identified as Giardia positive samples and were genotyped using glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi) and β-giardin loci.

Results

The three markers successfully genotyped 72 isolates and grouped 2 (1.4) is

SUBMITTER: Mbae C 

PROVIDER: S-EPMC4802924 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature