Sorting single satellite cells from individual myofibers reveals heterogeneity in cell-surface markers and myogenic capacity.

ABSTRACT: Traditional cell-screening techniques such as FACS and MACS are better suited for large numbers of cells isolated from bulk tissue and cannot easily screen stem or progenitor cells from minute populations found in their physiological niches. Furthermore, these techniques rely upon irreversible antibody binding, potentially altering cell properties, including gene expression and regenerative capacity. To address these challenges, we have developed a novel, label-free stem-cell analysis and sorting platform capable of quantifying cell-surface marker expression of single functional organ stem cells directly isolated from their micro-anatomical niche. Using our unique platform, we have discovered a remarkable heterogeneity in both the regenerative capacity and expression of CXCR4, β1-integrin,

SUBMITTER: Chapman MR 

PROVIDER: S-EPMC4803067 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature