Molecular Heterogeneity in Aldosterone-Producing Adenomas.

ABSTRACT:

Context

The use of next-generation sequencing has resulted in the identification of recurrent somatic mutations underlying primary aldosteronism (PA). However, significant gaps remain in our understanding of the relationship between tumor aldosterone synthase (CYP11B2) expression and somatic mutation status.

Objective

The objective of the study was to investigate tumor CYP11B2 expression and somatic aldosterone-driver gene mutation heterogeneity.

Methods

Fifty-one adrenals from 51 PA patients were studied. Immunohistochemistry for CYP11B2 was performed. Aldosterone-producing adenomas with intratumor CYP11B2 heterogeneity were analyzed for mutation status using targeted next-generation sequencing. DNA was isolated from CYP11B2-positive, CYP11B2-negative, and adjacent n

SUBMITTER: Nanba K 

PROVIDER: S-EPMC4803171 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature