Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission.

ABSTRACT: During anaphase, distinct populations of microtubules (MTs) form by either centrosome-dependent or augmin-dependent nucleation. It remains largely unknown whether these different MT populations contribute distinct functions to cytokinesis. Here we show that augmin-dependent MTs are required for the progression of both furrow ingression and abscission. Augmin depletion reduced the accumulation of anillin, a contractile ring regulator at the cell equator, yet centrosomal MTs were sufficient to mediate RhoA activation at the furrow. This defect in contractile ring organization, combined with incomplete spindle pole separation during anaphase, led to impaired furrow ingression. During the late stages of cytokinesis, astral MTs formed bundles in the intercellular bridge, but these failed to ass

SUBMITTER: Uehara R 

PROVIDER: S-EPMC4803307 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature