A strategy for dissecting the architectures of native macromolecular assemblies.

ABSTRACT: It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.

SUBMITTER: Shi Y 

PROVIDER: S-EPMC4803312 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature