Highly efficient recombinant production and purification of streptococcal cysteine protease streptopain with increased enzymatic activity.

ABSTRACT: Streptococcus pyogenes produces the cysteine protease streptopain (SpeB) as a critical virulence factor for pathogenesis. Despite having first been described seventy years ago, this protease still holds mysteries which are being investigated today. Streptopain can cleave a wide range of human proteins, including immunoglobulins, the complement activation system, chemokines, and structural proteins. Due to the broad activity of streptopain, it has been challenging to elucidate the functional results of its action and precise mechanisms for its contribution to S. pyogenes pathogenesis. To better study streptopain, several expression and purification schemes have been developed. These methods originally involved isolation from S. pyogenes culture but were more recently expanded to include rec

SUBMITTER: Lane MD 

PROVIDER: S-EPMC4803605 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature