Long-term time-lapse live imaging reveals extensive cell migration during annelid regeneration.

ABSTRACT:

Background

Time-lapse imaging has proven highly valuable for studying development, yielding data of much finer resolution than traditional "still-shot" studies and allowing direct examination of tissue and cell dynamics. A major challenge for time-lapse imaging of animals is keeping specimens immobile yet healthy for extended periods of time. Although this is often feasible for embryos, the difficulty of immobilizing typically motile juvenile and adult stages remains a persistent obstacle to time-lapse imaging of post-embryonic development.

Results

Here we describe a new method for long-duration time-lapse imaging of adults of the small freshwater annelid Pristina leidyi and use this method to investigate its regenerative processes. Specimens are immobilized with tetrodotoxi

SUBMITTER: Zattara EE 

PROVIDER: S-EPMC4804569 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature