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Microstructural heterogeneity directs micromechanics and mechanobiology in native and engineered fibrocartilage.


ABSTRACT: Treatment strategies to address pathologies of fibrocartilaginous tissue are in part limited by an incomplete understanding of structure-function relationships in these load-bearing tissues. There is therefore a pressing need to develop micro-engineered tissue platforms that can recreate the highly inhomogeneous tissue microstructures that are known to influence mechanotransductive processes in normal and diseased tissue. Here, we report the quantification of proteoglycan-rich microdomains in developing, ageing and diseased fibrocartilaginous tissues, and the impact of these microdomains on endogenous cell responses to physiologic deformation within a native-tissue context. We also developed a method to generate heterogeneous tissue-engineered constructs (hetTECs) with non-fibrous proteoglycan-rich microdomains engineered into the fibrous structure, and show that these hetTECs match the microstructural, micromechanical and mechanobiological benchmarks of native tissue. Our tissue-engineered platform should facilitate the study of the mechanobiology of developing, homeostatic, degenerating and regenerating fibrous tissues.

SUBMITTER: Han WM 

PROVIDER: S-EPMC4805445 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Microstructural heterogeneity directs micromechanics and mechanobiology in native and engineered fibrocartilage.

Han Woojin M WM   Heo Su-Jin SJ   Driscoll Tristan P TP   Delucca John F JF   McLeod Claire M CM   Smith Lachlan J LJ   Duncan Randall L RL   Mauck Robert L RL   Elliott Dawn M DM  

Nature materials 20160104 4


Treatment strategies to address pathologies of fibrocartilaginous tissue are in part limited by an incomplete understanding of structure-function relationships in these load-bearing tissues. There is therefore a pressing need to develop micro-engineered tissue platforms that can recreate the highly inhomogeneous tissue microstructures that are known to influence mechanotransductive processes in normal and diseased tissue. Here, we report the quantification of proteoglycan-rich microdomains in de  ...[more]

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