Mitigation of myocardial fibrosis by molecular cardiac surgery-mediated gene overexpression.

ABSTRACT:

Objective

Heart failure is accompanied by up-regulation of transforming growth factor beta signaling, accumulation of collagen and dysregulation of sarcoplasmic reticulum calcium adenosine triphosphatase cardiac isoform 2a (SERCA2a). We examined the fibrotic response in small and large myocardial infarct, and the effect of overexpression of the SERCA2a gene.

Methods

Ischemic cardiomyopathy was induced via creation of large or small infarct in 26 sheep. Animals were divided into 4 groups: small infarct; large infarct with heart failure; gene-treated (large infarct with heart failure followed by adeno-associated viral vector, serotype 1.SERCA2a gene construct transfer by molecular cardiac surgery with recirculating delivery); and control.

Results

Heart failure was signi

SUBMITTER: Katz MG 

PROVIDER: S-EPMC4805528 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature